Cell Bank Holdings

Mouse melanocytes

Mouse melanoblasts

Neural crest-like stem cells

Mouse melanoma

Human immortal melanocytes

Human melanoma lines

Mouse keratinocytes

Mouse fibroblasts

Producer cells

Mouse embryonal carcinoma

Mouse myoblasts

Rat pluripotent neuroblastoma and glial-like derivatives

Rat mammary myoepithelial

Rat mammary epithelial -glandular

Rat mammary epithelial -tumour (pluripotent)

Rat mammary fibroblasts

Notes

We are continually developing cell lines, especially melanocytes from additional mutant genotypes, and lines that are still under characterization may be available for collaborative projects. If you are interested in a genotype not listed here, please enquire.

* indicates a line to which access may be restricted or conditional. These are lines obtained from another group, lines requiring an MTA (material transfer agreement) or unpublished lines. Where unpublished lines are provided, it is generally on a collaborative basis. 

It would be valuable for future applications for funding renewals for the Cell Bank, if you could kindly acknowledge provision of cells by us when you publish any work using the cells, and cite the paper that describes the cells. Many thanks in advance if you can do this. If the cells were derived originally by another group, please show your courtesy by citing their paper.  If the reference is not yet entered here, we can provide it.

Mouse melanocyte lines

Cell Line

Mouse strain background

Medium

Reference

Nonagouti/black (a/a)

melan-a                              

C57BL/6J

1

Bennett, D.C. et al. Int. J. Cancer 39, 414-418 (1987).

melan-a2

C7BL/6J 

Sviderskaya, E.V. et al. Development 121, 1547-1557 (1995).

melan-a3 and a4 

C57BL/6J mixed 

Sviderskaya, E.V. et al. J. Natl. Cancer Inst. 94, 446-454 (2002). 

 melan-a5 to a7

strain a/a 

Hida, T. et al, Pigment Cell Melanoma Res. 22, 623-634 (2009).

DMEL-2* and DMEL-3*

C57BL/6J

3

Prince, S. et al. Cell Biol. Int. 25, 91-102 (2001).

DMEL-2 and DMEL-3 are engineered to express temperature-sensitive SV40 large T antigen. DMEL-3 is unpigmented at the permissive and pigmented at the nonpermissive (higher) temperature, except at higher passage levels (see Prince et al 2001). DMEL-2 is pigmented

Ink4a-Arf double null

Listed with wild-type cells because all tested immortal melanocyte lines fail to express p16Ink4a anyway, and often Arf. These lines were initially called melan-Ink4a(x) but this was changed to avoid confusion with Ink4a-only null cells. Many of the color-mutant lines below are also from Ink4a-Arf null mice.

melan-Ink4a-Arf1 to 4

C57BL/6J

2

Sviderskaya, E.V. et al. J. Natl. Cancer Inst. 94, 446-454 (2002)

p16Ink4a null, Arf competent (mouse genotype)

melan-Ink4a-1 to 4

C57BL/6J

8

Ha L et al. Proc. Natl. Acad. Sci. USA 104, 10968-10973 (2007).

Arf null, p16 competent (mouse genotype)

melan-Arf-1 and Arf-2

C57BL/6J

2

Ha L et al. Proc. Natl. Acad. Sci. USA 104, 10968-10973 (2007).

p53 (Tp53) null

melan-p53-1 to p53-3

C57BL/6J

2

Ha L et al. Proc. Natl. Acad. Sci. USA 104, 10968-10973 (2007).

Specific pigmentary genotypes - alphabetical by locus/mutation name

Agouti (A/A) [see above for black, a/a]

melan-A

129/Sv

2

Palmisano I et al., Hum. Mol. Genet. 17, 3487-3501 (2008). 

Mel-18*

(Tyr+,P+) C3H 

5

De Sepulveda, P. et al. Oncogene 9, 2655-2661 (1994). 

Mel-29*

C3H

5

De Sepulveda, P. et al. Oncogene 9, 2655-2661 (1994).

Agouti: dominant yellow (A^y/a)  

melan-Ay1 to Ay3

C57BL/6J

1 or 2

Hida T et al, Pigment Cell Melanoma Res. 22, 623-634 (2009).

Ashen (Rab27^ash/Rab27^ash)

melan-ash1 to ash3

C57BL/6J

2

Ali, B.R. et al. J. Cell Sci. 117, 6401-6412 (2004).

Brown (Tyrp1^b/Tyrp1^b)

melan-b

Q strain

5

Bennett, D.C. et al. Development 105, 379-385 (1989). 

melan-b-HmR 

Q strain

5 

Wakeling, W.F. et al. Br. J. Cancer 65, 529-537 (1992). 

melan-Ink4a-Arf-b1 to b3

C57BL/6J

2

Sviderskaya, E.V. et al. J. Natl. Cancer Inst. 94, 446-454 (2002).

Belted (Adamts20^bt/Adamts20^bt) 

melan-bt1* to bt3*

C57BL/6J

2

Unpublished

Albino (Tyr^c/Tyr^c)

melan-c

MF1

5

Bennett, D.C. et al. Development 105, 379-385 (1989).

Albino (Tyr^c-2J/Tyr^c-2J)

melan-c2* to c4*

C57BL/6J

2

Unpublished lines. c-2J is another functionally null allele.

Himalayan (Tyr^c-h/Tyr^c-h)

melan-c^h1* to c^h3*

C57BL/6J

2

Unpublished. Temperature-sensitive tyrosinase.

Chocolate (Rab38^cht/Rab38^cht)

melan-cht1 to 7

C57BL/6J

2

Wasmeier, C. et al. J. Cell Biol. 175, 271-281. 2006.

Cocoa (Hps3^coa/Hps3^coa)

melan-coa

C57BL/10J

2

Suzuki, T. et al. Genomics 78 , 30-37 (2001).

Dilute (d^l20J/d^l20J)

 melan-d1

 C57BL/6J

 2

Evans, R. et al. Curr. Biol. 24, 1743-1750 (2014)

ep, Pale ear (Hps1^ep/Hps1^ep)

melan-ep1 and ep2

C57BL/6J

2

Suzuki, T. et al. Nat. Genet. 30 , 321-324 (2002).

Chiang PW et al., J Biol. Chem. 278, 20332-7 (2003).

Grey - lethal (Ostm1^gl/Ostm1^gl)

melan-gl1* and gl2*

B6/129Sv

2

Unpublished

Gunmetal (Rabggta^gm/Rabggta^gm)

melan-gm1*

C57BL/6J

2

Unpublished

Light ear (Hps4^le/Hps4^le)

melan-le1 and le2

C57BL/6J

2

Suzuki, T. et al. Nat. Genet. 30, 321-324 (2002).

Chiang PW et al., J Biol. Chem. 278, 20332-7 (2003).

Leaden (Mlph^ln/Mlph^ln)

melan-ln1 to ln4

C57BL/6J

2

Hume, A.N. et al. Mol. Biol. Cell 17, 4720-4735 (2006).

Mahoganoid null (Mgrn1^md-nc/ Mgrn1^md-nc)

melan-md1 and md2

a/a mixed strain

1

Hida T et al, Pigment Cell Melanoma Res. 22, 623-634 (2009)

Mahogany null (Atrn^mg-3J/ Atrn^mg-3J)

melan-mg1 and mg2

a/a mixed strain

1

Hida T et al, Pigment Cell Melanoma Res. 22, 623-634 (2009).

Melanocortin 1 receptor (Mc1r^e/Mc1r^e) (extension)

melan-e1 to e3

C57BL/6J

1 +/- 40 pM CT

Hida T et al, Pigment Cell Melanoma Res. 22, 623-634 (2009).

Misty (Dock7^m/Dock7^m)

melan-m1 to m3

C57BL/6J

4

Sviderskaya, E.V. et al. Genetics 148, 381-390 (1998).

Mocha (Ap3d^mh/Ap3d^mh)

melan-mh1* and mh2*

C57BL/6J

2

Unpublished

Muted (Mu^mu/Mu^mu)

melan-mu1 to mu3

B6/CHMU/Le

2

Setty SRG et al. Mol. Biol. Cell. 18, 768-780 (2007).

Ocular albinism 1 KO (Oa1^-/- or Oa1^+/-)

melan-Oa1 

129/Sv (Tyr+,P+)

1

Palmisano I et al., Hum. Mol. Genet. 17, 3487-3501 (2008).

melan-Oa1+/-

129/Sv (Tyr⁺,P⁺)

1

Palmisano I et al., Hum. Mol. Genet. 17, 3487-3501 (2008).

Pallid (Pldn^pa/Pldn^pa)

melan-pa1 and pa2

C57BL/6J

2

Setty SRG et al. Mol. Biol. Cell. 18, 768-780 (2007).

Pearl (Ap3b1^pe/Ap3b1^pe)

melan-pe1

C57BL/6J

2

Theos, A.C. et al. Mol. Biol. Cell 16 , 5356-5372 (2005).

Pinkeyed dilution null (Oca2^p-cp/Oca2^p-25H)

melan-p1 and p2

C3H

7

Sviderskaya, E.V. et al. J. Invest. Dermatol. 108, 30-34 (1997).

Pinkeyed dilution null (Oca2^p-J/Oca2^p-J)

melan-p5* and p7*

C57BL/6J

2

Unpublished

Reduced pigmentation ( Rp^rp/Rp^rp)

melan-rp3 to rp5

C57BL/6J

2

Gwynn, B. et al. Blood 104, 3181-3189 (2004).

Recessive spotting (rs/rs)

melan-rs

C57BL/6J

1

Spanakis, E. Development 114, 675-680 (1992).

Piebald spotting (Ednrb^s/Ednrb^s)

melan-s1

C3H

2

Sviderskaya, E.V. Dev. Dyn. 213, 452-463 (1998).

Silver (Silv^si/Silv^si)

melan-si3 to si5

C57BL/6J

6

Theos, A.C. et al. Mol. Biol. Cell 17, 3598-3612 (2006).

Silver, heterozygous brown (Tyrp1⁺/Tyrp1^b, Silv^si/Silv^si)

melan-si1 and si2

C57BL/6J

6

Spanakis, E. Development 114 , 675-680 (1992).

Subtle grey (Slc7a11^sut/Slc7a11^sut)

melan-sut1 to sut3

B6/C3H/HeSnJ

4

Chintala, S. et al. Proc. Natl. Acad. Sci. 102, 10964-9 (2005).

Underwhite (Slc45a2^uw/uw)

melan-uw1 to uw3

 C57BL/6J

 2

 Le, L. et al. Mol. Biol. Cell. 31, 2687–2702 (2020)

Upstream factor (Usf KO)

melan-Usf1

mixed

2

Corre, S. et al. J. Biol. Chem. 279, 51226-51233 (2004).

Mouse melanoblast lines

Cell Line

Mouse strain background

Medium

Reference

Nonagouti/black (a/a)

melb-a

C57BL/6J

9

Sviderskaya, E.V. et al. Development 121, 1547-1557 (1995).

piebald spotting (Ednrb^s/Edrb^s)

melb-s1 to s3

C3H

9

Sviderskaya, E.V. Dev. Dyn. 213, 452-463 (1998).

pinkeyed dilution null (p^cp/p^25H)

melb-p3 and p4

C3H

10

Sviderskaya, E.V. et al. J. Invest. Dermatol. 108 , 30-34 (1997).

misty (m/m)

melb-m5

C57BL/6J

9

Sviderskaya, E.V. et al. Genetics 148 , 381-390 (1998).

Neural crest-like cell lines

Cell Line

Mouse Strain Background

Medium

Reference

misty (m/m)

NC-m4* and NC-m6*

C57BL/6J

11

Sviderskaya, E.V. et al., FASEB J 23, 3179-3192 (2009).

Mouse melanoma cell lines

Cell Line

Mouse strain background

Medium

Reference

B16-C3 

C57BL/6J 

12

Kreider, J.W. et al., J. Natl. Cancer Inst. 54, 1457-1467 (1975).

B16-C3.3 

C57BL/6J 

12

Bennett, D.C. Cell 34, 445-453 (1983).

B16 C3.6

C57BL/6J 

12

Bennett, D.C. Cell 34, 445-453 (1983). 

B16-F1* 

C57BL/6J 

12

Fidler, I.J. Cancer Res. 35 , 218-224 (1975). 

B16-F10* 

C57BL/6J 

12

Fidler, I.J. Cancer Res. 35 , 218-224 (1975). 

K1735-m2*

C3H/HeN

12

Fidler, I.J. et al, J. Natl. Cancer Inst. 67, 947-956 (1981)

Human immortal melanocytes

These cells are subject to a material transfer agreement. They are grown under low-level biohazard containment conditions as they contain disabled amphotropic viruses used in the immortalization procedure.

Cell Line

Medium

Reference

Hermes 1* 

8

Sviderskaya, E.V. et al. J. Natl. Cancer Inst. 95, 723-732 (2003). 

Hermes 2*[animal experiments with this line not permitted]

8

Sviderskaya, E.V. et al. J. Natl. Cancer Inst. 95, 723-732 (2003).

Hermes 1 and Hermes 2 (sublines) are p16 null melanocytes immortalized with hTERT (puro vector) only. Hermes 1 is immortalized from primary strain Hmel-p16-1, and Hermes 2 from Hmel-p16-2. Sublines: Hermes 2A (slighly aneuploid), Hermes 2B and 2C (both diploid). 

Hermes 3*

8

Gray-Schopfer, V.C. et al. Br. J. Cancer 95, 496-505 (2006).

Immortalized lines from Nohm-1 normal diploid human melanocyte strain. Sublines: Hermes 3A has hTERT (puro) and CDK4 (neo) expression vectors. Hermes 3B has hTERT (puro) and antisense p16 (neo) vectors. Hermes 3C has hTERT (puro) and HPV16-E7 (neo) vectors. 

Hermes 4*

8 except 2 pM CT

Gray-Schopfer, V.C. et al. Br. J. Cancer 95, 496-505 (2006).

Immortalized lines from 830c normal diploid melanocytes. (830c melanocytes carry two variant MC1R alleles: R160W/D294H, see MC Scott et al, J. Cell Sci. 115, 2349, 2002). Sublines: Hermes 4A has hTERT (puro) and CDK4 (neo) expression vectors. Hermes 4B has hTERT (puro) and antisense p16 (neo) vectors. Hermes 4C has hTERT (puro) and HPV16-E7 (neo) vectors. 

Human melanoma cell lines (metastatic unless stated)

(*) indicates a line to which access may be restricted or conditional. These are lines obtained from another group, lines requiring an MTA (material transfer agreement) or unpublished lines. Where unpublished lines are provided, it is generally on a collaborative basis.

Cell Line

Medium

Reference

A375P (primary lesion, metastatic in mice)

13

Brüggen, J. et al. Cancer Immunol. Immunother. 5 , 53-62 (1978). 

A375M (highly metastatic in mice) 

13

Kozlowski, J.M. et al. Cancer Res. 44, 3522-3529 (1984).

COLO 697* 

Easty, D.J. et al. Cancer Res. 55, 2528-2532 (1995).

COLO 792* 

Easty, D.J. et al. Cancer Res. 55, 2528-2532 (1995). 

COLO 793* 

Unpublished

COLO 800* 

Easty, D.J. et al. Cancer Res. 55, 2528-2532 (1995). 

COLO 818* 

Easty, D.J. et al. Cancer Res. 55, 2528-2532 (1995).

COLO 832* $ 

Easty, D.J. et al. Cancer Res. 55, 2528-2532 (1995). 

COLO 839* $ In-transit - from peripheral blood) 

Morse, H.G. & Moore, G.E. Cancer Genet. Cytogenet. 69, 108-112 (1993). 

COLO 845* $ 

Easty, D.J. et al. Cancer Res. 55, 2528-2532 (1995).

COLO 857* $ 

13

Easty, D.J. et al. Cancer Res. 55, 2528-2532 (1995).

COLO 858* $ $ = lines from different secondary sites from the same patient.

13

Maung, K., et al. Oncogene 18, 6824-6828 (1999).

DX3* (Parental, metastatic) 

13

Dracopoli, N.C. et al. Cancer Res. 47, 3995-4000 (1987).

DX3-LT5.1* (Highly metastatic in mice) 

13

Ormerod, E.J. et al. Cancer Res. 46, 884-890 (1986). 

LT5.1 Hm^R*(hygromycin-resistant)

13

Unpublished subline, derived at SGUL.

ME 1402* (primary, VGP) 

13

Larizza, L. et al. Clin. Exp. Metastasis 7, 633-644 (1989).

ME 10538* (primary, VGP) 

13

Larizza, L. et al. Clin. Exp. Metastasis 7, 633-644 (1989).

MM96*TgR (thioguanine-resistant subline of MM96) 

13

Pope, J.H. et al. Pathology 11, 191-195 (1979). 

MM200* (primary) 

13

Pope, J.H. et al. Pathology 11, 191-195 (1979). 

MM485*

13

McEwan, M. et al. Pigment Cell Res. 2, 1-7 (1989).

RPMI 7932* 

Quinn, L.A. et al. J. Natl. Cancer Inst. 59, 301-307 (1977). 

SGM2 (primary, RGP) 

Soo et al. Pigm. Cell Melanoma Res. 24, 490-503 (2011). 

SGM3 (primary, RGP) 

Soo et al. Pigm. Cell Melanoma Res. 24, 490-503 (2011). 

SGM4 (primary, RGP) 

Soo et al. Pigm. Cell Melanoma Res. 24, 490-503 (2011). 

SGM5 (primary, VGP)

Soo et al. Pigm. Cell Melanoma Res. 24, 490-503 (2011).

SK-MEL-23* 

13

Houghton, A.N. et al. J. Exp. Med. 164 , 812-829 (1987).

SK-MEL-23-neoR (G418-resistant) 

13

Unpublished subline. 

WM-9* 

13

Balaban, G. et al. Cancer Genet. Cytogenet. 11 , 429-439 (1984).

WM-9- Hm^R

13

Hm^R is unpublished subline.

WM-35* (primary, RGP) 

13

Herlyn, M. Cancer Metastasis Rev. 9, 101-112 (1990).

WM-98-1* (primary, VGP) 

13

Herlyn, M. et al. Cancer Res. 45, 5670-5676 (1985).  

WM-239A* 

13

Herlyn, M. et al. J. Natl. Cancer Inst. 74 , 283-289 (1985).

WM-239A-Hm^R

13

Unpublished subline.

WM-852* 

13

Herlyn, M. et al. Adv. Cancer Res. 54, 213-234 (1990). 

WM-1158* 

13

Easty, D.J. et al. Int. J. Cancer 60, 129-136 (1995). 

WM-1341B* (primary, VGP) 

13

Herlyn, M. Cancer Metastasis Rev. 9, 101-112 (1990). 

WM-1650* (primary, RGP) 

2

Easty, D.J. et al. Int. J. Cancer 60, 129-136 (1995). 

451LU* (more-metastatic subline of WM-164)

13

Herlyn, D. et al. Cancer Res. 50, 2296-2302 (1990).

Mouse keratinocytes

Cell Line

Medium

Reference

XB2

14

Rheinwald JG, Green H. Cell 6, 317-330 (1975).

Mouse fibroblasts

Cell Line

Medium

Reference

SC1 dermal 

13

Sviderskaya EV et al. Development 121 , 1547-1557 (1995).

Swiss 3T3 

14

STO-SF B82* 

1

LTK-Silverstein* 

14

 Pellicer, A. et al.  Cell 14:133-41, (1978)

Fibro-Ink4a-Arf 

14

Sviderskaya, E.V. et al. J. Natl. Cancer Inst. 94, 446-454 (2002)

Dermal (Ink4a-Arf null)

14

Sviderskaya, E.V. et al. J. Natl. Cancer Inst. 94, 446-454 (2002)

Fibro-Ay (A^y/A, believed to secrete ASP)

14

Hida T et al, Pigment Cell Melanoma Res. 22, 623-634 (2009).

Retroviral producer cells

Cell Line

Medium

Reference

GP+E*

14

GP+E-Kit* 

14

Y 2 [psi-2]* 

14

Y 2-Tyrp1* 

14

Phoenix-ECO*

14

Mouse embryonal carcinoma

Cell Line

Medium

Reference

P19

15

 Jones-Villeneuve, E.M. et al., J Cell Biol. 94(2):253-262. (1982).

Mouse myoblasts

Cell Line

Medium

Reference

C2 MM14* 

16

MM14 DR* 

17

FAF1 

FAF2 

FAF3 

FAF4 

12

FAF2-CAPR

12

Slow-fusing SLOF1

12

Rat pluripotent neuroblastoma and glial-like derivatives

Cell Line

Medium

Reference

RT4-AC* 

18

Freeman, MR. and Sueoka, N.,  Proc Natl Acad Sci USA, 84:5808-12 (1987).

RT4-AC23* 

18

Freeman, MR. and Sueoka, N., Proc Natl Acad Sci USA, 84:5808-12 (1987).

RT4-AC24* 

18

Freeman, MR. and Sueoka, N., Proc Natl Acad Sci USA, 84:5808-12 (1987).

RT4-E5* 

18

Freeman, MR. and Sueoka, N., Proc Natl Acad Sci USA, 84:5808-12 (1987).

RT4-36A*

18

Freeman, MR. and Sueoka, N., Proc Natl Acad Sci USA, 84:5808-12 (1987).

Rat mammary myoepithelial

Cell Line

Medium

Reference

M1 Rama 3 to 6, 12, 15, 16, 18, 29 to 31, 43, and 44

14

Bennett DC et al., Cell 15 , 283-298 (1978).

Rat mammary epithelial-glandular

Cell Line

Medium

Reference

 Rama 39 to 41 

19

Rat mammary epithelial-tumour (pluripotent)

Cell Line

Medium

Reference

 Rama 22, 25, 26, 32 to 35, 37 and 45

19

Bennett DC et al., Cell 15 , 283-298 (1978).

Rat mammary fibroblasts

Cell Line

Medium

Reference

 Rama 13, 14, 19, 29 and 27

14

Bennett DC et al., Cell 15 , 283-298 (1978).